The Bioenergetics Core occupies approximately 2,500 sq. ft. within the Institute for Nanoscience and Engineering (NANO 240). It provides access, training, and assistance with live-cell metabolic assay platforms.
Key Equipment Contained in the Bioenergetics Core:
Measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format. High-sensitivity analysis of as few as 5,000 cells per well using the custom 96-well plate.
Measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 24-well plate format. High sensitivity to analyze as few as 10,000 cells per well in the custom 24-well plate.
Measure mitochondrial respiration and glycolysis as well as the ATP production rate of live cells in an eight-well mini-plate format.
The Seahorse models are connected to a BioTek cell imager that can facilitate the detection of discrete changes in cellular bioenergetics in real-time. More importantly, real-time cell measurements provide a clear window into the critical functions driving cell signaling, proliferation, activation, toxicity, and biosynthesis.
Cell or tissue measurements of oxygen consumption, H2O2 production, ATP production, Ca2+, and mitochondrial membrane potential.
A cell imaging module built directly into a reader, enabling automated cell counting, viability analysis in slides, bright field imaging, and automated confluence assessment in microplates.
A power-packed solution that delivers fast, efficient separations from samples as small as 175 μL up to 32.4 ml and at speeds of up to 150,000 RPM and more than 1,000,000 x g.
The Bioenergetics Core also provides access to biological safety cabinets, controlled temperature incubators (C02 and non), preparative ultracentrifuge (to isolate sub-cellular organelles), a floor model high-speed refrigerated centrifuge, a Savant Speed Vac, Trinocular microscope, NGC Purification System, AKTA go FPLC, shaking water bath, UV Vis Spectrophotometer, Mastercycler, Electrophoresis equipment, Western Blot, Milli-Q water purification, hotplate and stirrer, balance, and freezers to assist with conducting experiments on-site.
Please contact our Bioenergetics Core Director or Core Manager to schedule access to the equipment, or discuss experiments we can support.
Core Director
Dr. Suresh Thallapuranam
Mildred-Cooper Endowed Chair of Biochemistry & Bioinformatics
Chemistry and Biochemistry
Fulbright College of Arts & Sciences
University of Arkansas
sthalla@uark.edu
Profile
Core Manager
Dr. Patience Okoto
Research Associate
Chemistry and Biochemistry
Fulbright College of Arts & Sciences
University of Arkansas
psokoto@uark.edu